Cochlear explant
WebJun 8, 2024 · The cochlear explant serves as an essential experimental tool in hearing research (Landegger et al., 2024b) because it offers organotypic 3D cultures for a facilitated in situ cellular evaluation ... WebHere we present the preparation, culture, treatment, and immunostaining of neonatal murine cochlear explants. The careful preparation of these explants facilitates the identification of mechanisms that contribute to …
Cochlear explant
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WebThe application of HGF to cochlear explant cultures significantly reduced the hair cell loss induced by neomycin. Immunohistochemistry showed c-Met expression in normal auditory hair cells, and its increase in response to neomycin-induced damage. WebCochlear implant assessment. Assessment process; Talking to your audiologist; Wearing cochlear implants from different manufacturers; Insurance and cost issues; Applying for …
WebVestibular schwannoma (VS) is a tumor of the vestibular nerve that transmits balance information from the inner ear to the brain. Sensorineural hearing loss occurs in 95% of patients with these tumors, but the cause of this loss is not well WebFeb 3, 2024 · Cochlear implants are electronic hearing devices. Doctors implant cochlear implants into people with severe to profound hearing loss to produce useful hearing sensations. The FDA regulates...
WebAug 21, 2024 · Cochlear hair cell loss is a leading cause of deafness in humans and other mammals. In the immature cochlea, lost hair cells are regenerated by neighboring glia-like supporting cells. However, for reasons unknown, such regenerative capacity is rapidly lost as supporting cells undergo maturation. WebDec 4, 2024 · To overcome this limitation and effectively study reprogramming and proliferation in adult cochleae, we established a system of adult whole cochlea explant culture (Supplementary Fig. 2 ). This...
WebAbstract. Objectives: Removal of a cochlear implant and its intracochlear electrode array is sometimes necessary, potentially causing cochlear explant trauma. Explantation …
WebPlace the cochlear explants in prepared blocking solution (94% PBS, 5% NHS, and 1% non-ionic surfactant) for 30 min on shaker. Place the cochlear explants in a prepared stock antibody solution (98.6% PBS, 1% NHS, and 0.4% non-ionic surfactant) with the respective primary antibodies (vortex before use) O/N on a counter at RT. barbara titusWebExplantation typically occurs years post-implantation by which time reactive tissue has formed around the electrode. We aimed to create an in-vitro electrode explant model to examine explant forces and intracochlear trauma across multiple electrode types and insertion depths. barbara tkotzWebNational Center for Biotechnology Information barbara toalWebNov 1, 2002 · Cochlear explant cultures. Cochlear explant cultures were prepared as described previously ( Kelley et al., 1993 ; Raz and Kelley, 1999 ). Briefly, timed pregnant ICR strain mice were anesthetized deeply by the inhalation of CO 2 gas and then killed by creating a double pneumothorax on E13 or E14. barbara to therapistWebDevelopment of the cochlea occurs within the temporal bone of the skull making visualization or perturbation of this process challenging. One approach to address this … barbara toanWebTo study neomycin uptake in the hair cells, cochlear explants were incubated with Neomycin Texas-red (NTR) conjugate. As expected, exposure to neomycin significantly reduced the survival of inner (IHC) and outer hair cells (OHC). IHC survival rate was high in the apical segment and low in the basal segment. barbara tobelaimWebAug 23, 2016 · Within each replicate, control and experimental cochlear explant electroporations were performed on litter-mate cochleae with developmental stage determined using the Theiler staging criteria 26. All cell counts were performed using image J and statistics were performed using Graph Pad Prism statistical software (Graph Pad). barbara toates