Corynebacterium pcr

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August undefined, 2024

WebNov 5, 2024 · This chapter will address the main omics approaches used in studies involving the genus Corynebacterium, Gram-positive microorganisms that can be isolated from many diverse environments. Currently, the genus Corynebacterium has more than 130 highly diversified species, many of which present medical, veterinary and … WebDetection of toxin-producing Corynebacterium diphtheriae from throat swabs of diphtheria patients using duplex real-time PCR Detection of toxin-producing Corynebacterium diphtheriae from throat swabs of diphtheria patients using duplex real-time PCR Iran J Microbiol. 2024 Dec;12 (6):508-515. doi: 10.18502/ijm.v12i6.5024. Authors

Rapid detection and discrimination of potentially toxigenic ...

WebDec 16, 2016 · To decrease the time to result, a real-time PCR (qPCR) assay was developed for confirmation of both identification of Corynebacterium diphtheriae and Corynebacterium ulcerans/Corynebacterium pseudotuberculosis and detection of the diphtheria toxin gene. Target genes were the RNA polymerase β-subunit-encoding gene … WebJul 29, 2024 · The diphtheria toxin gene target in the PCR used is sensitive to approximately 10 genome copies per reaction, while the rpoB targets for C. diphtheriae and C. ulcerans/C. pseudotuberculosis are ... myhub oracle

Construction and application of a CRISPR/Cas9-assisted

WebJan 7, 2024 · Corynebacterium tuberculostearicum ( C. t .) is a ubiquitous bacterium that colonizes human skin. In contrast to other members of the genus Corynebacterium, such as toxigenic Corynebacterium diphtheriae or the opportunistic pathogen Corynebacterium jeikeium, several studies suggest that C. t. may play a role in skin health and disease. WebJun 5, 2014 · The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve … WebApr 1, 2024 · We developed a multiplex real-time PCR assay with amplicon melting curve analysis to rapidly discriminate Corynebacterium ulcerans from Corynebacterium pseudotuberculosis and detect the bacterial diphtheria toxin gene. This assay should be a valuable tool for identification of potentially toxigenic C. ulcerans. myhub.nttdataservices.com my links navigator

Identification and molecular characterization of Corynebacterium ...

Corynebacterium - an overview ScienceDirect Topics

WebJan 10, 2024 · Corynebacterium species are Gram-positive bacteria which frequently colonize the skin and upper airway. While Corynebacterium diphtheriae, the causative agent of diphtheria, is an important pathogen ( Bernard, 2012 ), other Corynebacterium species are rarely associated with disease. WebDec 1, 2006 · Corynebacterium, PCR, divIVA, ARDRA, 16S rRNA gene, real-time PCR Issue Section: Research Letters Introduction The genus Corynebacterium includes a … ohio water polo associationWebMay 21, 2015 · Corynebacterium urealyticum (formerly known as coryneform CDC 79 group D2) was first recognized to be involved in human infections 80 years ago. It is a … my hub nottingham

"WebAll the 45 isolates were 100% susceptible to vancomycin and linezolid, but highly resistant to macrolide–lincosamide–streptogramin B (MLSB), fluoroquinolones, tetracyclines and β-lactams with corresponding mechanisms. The detection rate of multidrug–resistant (MDR) non-diphtheriae Corynebacterium is 91.1%. All isolates are able to form ... " - Corynebacterium pcr

Corynebacterium pcr

Detection and Characterization of Diphtheria Toxin Gene …

WebJan 1, 2024 · The genus Corynebacterium is a member of the class Actinobacteria (high G+C Gram-positive bacilli), subclass Actinobacteridae, order Actinomycetales, suborder … WebAll available Corynebacterium rpoB sequences were analyzed by computer- assisted restriction analysis. The rpoB PCR-RFLP pattern predicted by using endonucleases MseI and NlaIV clearly differentiated C. striatum from all other Corynebacterium species.

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WebOct 5, 2024 · Based on the predominance of Corynebacteria with the absence of other bacteria on Gram stain and a negative PCR for respiratory viruses, his pneumonia was attributed to C. propinquum, and only vancomycin was continued. He responded and was discharged to complete a 10-day course of linezolid. Fig. 1 WebCorynebacterium bovis PCR Kit Technique PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values …

WebApr 1, 2024 · A real-time PCR assay was developed for detection of putative toxigenic Corynebacterium ulcerans. This assay could rapidly discriminate C. ulcerans from … WebThe Corynebacterium is a genus of bacteria of growing clinical importance. Progress in medicine results in growing population of immunocompromised patients and growing …

WebFeb 23, 2024 · 5-Aminolevulinic acid (5-ALA) has recently received much attention for its wide applications in medicine and agriculture. In this study, we investigated the effect of NCgl0580 in Corynebacterium glutamicum on 5-ALA biosynthesis as well as its possible mechanism. It was found that the overexpression of NCgl0580 increased 5-ALA … WebCorynebacterium ( / kɔːˈraɪnəbækˌtɪəriəm, - ˈrɪn -/) is a genus of Gram-positive bacteria and most are aerobic. They are bacilli (rod-shaped), and in some phases of life they are, more specifically, club -shaped, which inspired the genus name ( …

WebMay 19, 2024 · Corynebacterium glutamicum is widely used as microbial cell factory for various bioproducts, but its genomic editing efficiency needs to be improved. In this study, a highly efficient CRISPR/Cas9-assisted genomic editing system for C. …

WebCorynebacterium spp.棒杆菌通用染料法荧光定量PCR试剂盒，本产品仅供科研，不可做临床诊断使用。. 如需更详细的完整说明书，可联系客服添加微信或QQ免费索取。. 本产品是以染料法荧光定量PCR技术为基础开发的PCR试剂盒，它具有下列特点：. 1.即开即用，用户只 ... ohio waterfront land for saleWebSep 1, 2024 · The conventional PCR can be used at the state laboratories for rapid detection of toxin genes in toxigenic C. diphtheriae cultures, thus early treatment can be given to the patients while waiting ... my hub palletwaysWebSep 3, 2024 · Introduction. Diphtheria is caused by toxigenic strains of Corynebacterium diphtheriae , Corynebacterium ulcerans and Corynebacterium pseudotuberculosis . … my hub parentsWebAug 11, 2024 · Corynebacterium glutamicum (C. glutamicum) is widely used for the production of L-amino acids, vitamins, organic acids, fuel ethanol and other products 1,2,3, and manipulation of its genes is ... myhub phoneWebApr 1, 2024 · A real-time PCR assay was developed for detection of putative toxigenic Corynebacterium ulcerans. • This assay could rapidly discriminate C. ulcerans from Corynebacterium pseudotuberculosis. • This assay successfully detected tox genes of C. ulcerans and Corynebacterium diphtheriae. • myhub peoplexdWebPCR and sequencing has been used for further identification (Greenwood, 2007; Hawkins et al., 2009). Prevention and Therapy Many Corynebacterium spp. have significant … myhub rewardsWebJan 16, 2024 · Twenty Corynebacterium strains were isolated and identified as Corynebacterium striatum (n = 7), Corynebacterium amycolatum (n = 7), C. urealyticum (n = 3), Corynebacterium afermentans (n = 2), and Corynebacterium pseudodiphtheriticum (n = 1). ... PCR conditions consisted of an initial activation at 94 °C … myhub pearson