WebiQ™ SYBR ® Green Supermix, 100 x 50 µl rxns, 2.5 ml (2 x 1.25 ml) from Bio-Rad Be the first to write a review! Citations: (223) Use iQ SYBR ® Green Supermix with fluorescein in real-time PCR detection systems requiring a passive reference dye for well factor collection. Detect as few as 10 copies of template WebItem iQ™ SYBR ® Green Supermix. Company Bio-Rad. Catalog Number 170-8882.
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WebThese reactions were performed using IQ SYBR Green Supermix (Bio-Rad, Hercules, CA, USA) with the CFX96 Real-Time System (Bio-Rad), as described previously . ... Each reaction contained: 7.5 μL of the iQ SYBR Green Supermix, (BioRad) 200 nM of forward and reverse primers, and 0.3 μL cDNA, to a final volume of 15 μL. ... Weba SYBR Green SuperMix 95054 -500, 95054-02K Bio-Rad CFX96 ™ , CFX384 ™ , Opticon™, MiniOpticon™, Chromo4™ Cepheid Smart Cycler ®;Qiagen/Corbett Rotor-Gene Eppendorf Mastercycler ® ep realplex Roche Applied Science LightCycler ® 480 . Components . PerfeC. T. a SYBR Green SuperMix (2X): 2X reaction buffer containing optimized ...
WebJul 24, 2024 · Quantitative real-time PCR (qPCR) was performed using iQ SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) on a Bio-Rad iCycler using β-actin as control, and data were analyzed using the ∆∆ C t method . All primer sequences used in this study are listed in Table 1. WebReal-time PCR primer assay designed for SYBR ® Green gene expression analysis. Info: Recommended - best coverage; Same primer pair as used in probe assay …
WebiTaq Universal SYBR Green Supermix contains: Advanced buffer formulation with SYBR Green I for optimal qPCR performance; Antibody-mediated hot-start iTaq DNA polymerase … WebApr 20, 2010 · iQ SYBR Green Real-Time PCR Supermix from BioRad. Bio-Rad offers the iQ SYBR Green Supermix which contains dNTPs, 50 U/ml iTaq DNA polymerase, 6 mM MgCl …
Select the best real-time PCR supermix for your application: 1. Bio-Rad’s Universal Supermixes can be used in all real-time PCR systems — both ROX … See more
WebJun 28, 2016 · RNA (2 µg) was used for first-strand cDNA synthesis with reverse transcriptase (Promega Corporation, Madison, WI, USA), according to the manufacturer's protocol. cDNA was amplified by qPCR in a CFX96 Touch Real-Time PCR Detection system with CFX Manager 3.0 software (Bio-Rad Laboratories, Inc., Hercules, CA, USA) using iQ … bwitch bra online shoppingWebFeb 24, 2012 · The iTaq™ universal SYBR ® Green supermix and iTaq™ universal probes supermix are ready-to-use 2x supermixes formulated for sensitive, efficient, and reproducible qPCR data on any instrument. iTaq universal supermixes let you: Obtain reliable and reproducible gene expression analysis results c# falsy valuesWebMay 21, 2012 · Finally, we titered the SYBR Green I concentrations in standard PCR master mixes and compared the normalized expression of the genes icaA, bhp, aap, psmβ1 and agrB using 4 distinct biofilm forming S. epidermidis strains to the results obtained with commercially available kits. bwit 100WebDescription. PerfeCTa qPCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers, probe (s), and template for real-time … b-wit 100 wideband injection transformerWebAug 10, 2011 · The iQ™ SYBR ® Green Supermix contains 3 mM MgCl 2. 5'-nuclease probe hydrolysis reactions consisted of 0.4 U iTaq DNA polymerase (BIO-RAD, Hercules, CA), 1 μL 10X iTaq™ buffer (BIO-RAD, Hercules, CA), 3 mM MgCl 2, 800 μM dNTPs, and 200 nM each of the forward and reverse primers and the probe, 20 to 2 × 10 6 copies of the synthetic … bwi taxi ratesWebAug 25, 2015 · Real-time PCR was performed using the iQ SYBR Green Supermix kit (Bio-Rad), with the following temperature cycles: cycle 1 pre-denaturation (10 min, 95 °C), cycle 2 repeated 50 times: denaturation (30 s, 95 °C), annealing (30 s at the annealing temperatures— T a, indicated in Table 2), and elongation (45 s, 72 °C) steps and cycle 3 … cfa lynchburgWebiTaq Universal SYBR Green Supermix is a 2x concentrated, ready-to-use reaction master mix optimized for dye-based quantitative PCR (qPCR) on any real-time PCR instrument. It contains antibody-mediated hot-start iTaq DNA polymerase, dNTPs, MgCl 2 , SYBR Green I dye, enhancers, stabilizers, and a blend of passive reference dyes (including ROX and ... bwi taxi service