Lamp2b
Tīmeklis2024. gada 11. jūl. · The Lamp2b insert was then ligated into pmEGFP-C1 (Addgene) backbone with the unique restriction sites NheI and BamHI, removing eGFP in the process. TīmeklisPlasmid pcDNA GNSTM-3-Flag-10-Lamp2b-HA from Dr. Joshua Leonard's lab contains the insert Lamp2b and is published in J Biol Chem. 2015 Mar 27;290(13):8166-72. doi: 10.1074/jbc.M114.621383. Epub 2015 Feb 5. This plasmid is …
Lamp2b
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TīmeklisCD51 + CD61 (Integrin alpha V + Integrin beta 3) CD51 / Integrin alpha V. CD52 Tīmeklis2011. gada 20. marts · Lamp2b was cloned with cDNA from C2C12 cells and XhoI and BspEI restriction sites were inserted after the signal peptide sequence together with glycine linkers using primers listed in ...
Tīmeklis利用本发明所提供的融合蛋白制备的靶向EGFR脑转移的外泌体(GE11‑Exo),通过lamp2b确保了融合蛋白在外泌体膜表面定位,利用抗EGFR靶向肽GE11对外泌体进行表面修饰来降低脱靶效应,具有特异结合EGFR的主动靶向能力。电子显微镜、粒径分析仪、western blot法检测GE11 Tīmeklis利用携带软骨细胞的亲合肽(CAP)融合表达展示于外泌体膜蛋白(lamp2b)上表面作为靶向的基因工程化外泌体可实现靶向软骨细胞(图1)。 与无靶向的天然外泌体相比,关节内注射的CAP外泌体在能长时间保留在关节腔中,减少体内扩散。
Tīmeklis2024. gada 1. febr. · Plasmid CAP-lamp2b includes a glycosylation sequence (GNSTM), a E7 sequence (EPLQLKM) and glycine-serine spacers at the N-terminus of lamp2b … Tīmeklis研究mirna的靶点mrna,构建过表达质粒,是否需要考虑3‘utr区,还是只过表达cds区就行了?. rt,因为mirna和mrna的3’utr区结合,过表达是仅过表达cds区是不是没有效果?是不是要包含3‘utr区?. 丁香园-核酸基因技术-2024-05-03 09:48:59
TīmeklisDownload scientific diagram Targeting peptide expressed with Lamp2b is expressed on the external surface of exosomes. (a) Schematic representation of production, harvest and re-administration of ...
Tīmeklis2011. gada 20. marts · FLAG-Lamp2b was strongly expressed in dendritic cells and was incorporated into the dendritic cell–derived exosomes based on western blots with anti-FLAG and anti-Lamp2b antibodies . As the ... kids club hotel resorts floridaTīmeklis2024. gada 23. maijs · The expression of CTP-Lamp2b peptide on exosomes was stabilized by attaching glycosylation sequences. Exosomes expressing only Lamp2b … is milk hypertonicTīmeklisSynthetic peptide corresponding to Human LAMP2B aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. LAMP2 has 3 distinct isoforms, … is milk high in potassiumTīmeklisЭкзосомы обнаружены в различных тканевых жидкостях организма, таких как сыворотка крови [9], спинномозговая жидкость, а также в моче [10], слюне и грудном молоке [11] . Функции экзосом ... is milk homogeneous mixtureTīmeklis2024. gada 22. apr. · Constructing Lamp2b–iRGD: The RVG fragments at both ends of the Lamp2b-RVG plasmid (i.e., enzyme digestion sites BspEI and XhoI) were replaced with iRGD peptides, single-stranded sequences synthesized through annealing of the restriction sites. Then, the sticky ends were, respectively, annealed with a forward … is milk healthy for a dietTīmeklis2024. gada 21. marts · LAMP2 (Lysosomal Associated Membrane Protein 2) is a Protein Coding gene. Diseases associated with LAMP2 include Danon Disease and Left … kids clubhouses backyardTīmeklis1.一种用于肿瘤放疗增敏的工程化外泌体,其特征在于,所述用于肿瘤放疗增敏的工程化外泌体外部修饰肿瘤靶向肽,内部包裹小干扰RNA核酸药物。 2.根据权利要求1所述的用于肿瘤放疗增敏的工程化外泌体,其特征在于,所述核酸药物是长度为15‑30个核苷酸。 3.根据权利要求2所述的用于肿瘤放疗增 ... is milk high in protein