Optical fan for single-cell screening
WebFeb 15, 2024 · Single-molecule fluorescence in situ hybridization: a method where several fluorescent oligonucleotides are bound to individual DNA or RNA molecules, allowing them to be identified and counted... WebAug 1, 2024 · Optical fan for single-cell screening. J. Biophot., 13 (2024), Article e202400155, 10.1002/jbio.202400155. View in Scopus Google Scholar [22] Y. Xie, H. Bachman, T.J. Huang. Highly efficient single cell arraying by integrating acoustophoretic cell pre-concentration and dielectrophoretic cell trapping.
Optical fan for single-cell screening
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WebJan 19, 2024 · An optical approach for cell enrichment by patterned illumination followed by FACS sorting We developed an approach, which we term optical enrichment, to select cells of interest using a microscope and mark them by photoactivation, enabling cell isolation using FACS ( Fig. 1 ). WebNov 29, 2024 · The target cell is then captured by a 1064 nm optical tweezer and deposited as one-cell-harboring nanoliter microdroplets in a one-cell-one-tube manner. For bacterial, yeast and human cells, OPSI achieves a >99.7% target-cell sorting purity and a 10-fold elevated speed of 10–20 cells per min.
WebThe single-cell screening has attracted great attentions in advanced biomedicine and tissue biology, especially for the early disease diagnosis and treatment monitoring. In this work, by using a specific-designed fiber probe with a flat facet, we propose an "optical fan" strategy to screen K562 cells at the single-cell level from a populations of RBCs. After the 980-nm … WebLiu, X., Li, Y., Xu, X., Zhang, Y., & Li, B. (2024). Optical fan for single‐cell screening. Journal of Biophotonics. doi:10.1002/jbio.202400155
WebAug 13, 2024 · MR: One of the major challenges in single-cell analysis is the initial isolation and culture of a single cell. Cell viability is required for downstream analysis and further cell outgrowth. However, cells are not usually on their own and can be difficult to culture independently. Cells are heavily influenced by their surrounding environment and ... WebThe single‐cell screening has attracted great attentions in advanced biomedicine and tissue biology, especially for the early disease diagnosis and treatment monitoring.
WebFeb 10, 2024 · The use of sophisticated models such as organoids and whole organisms, flexible perturbations such as gene activation and repression, diverse biological challenges and data-rich read-outs such as...
Webcell line.19 Imaging flow cytometry has succeeded by combining the optical characterization of cell size and morphology,9 ,2024 refractive index,1112 or deformability,22−24 with proven capability for label-free cell screening in leukemia diagnosis.25 A more recent route in label-free cytometry proposes the use of mechanical … iron oxygen evolution reactionWebFeb 24, 2024 · We set out to create single-chain optically controllable MEK1 using pdDronpa1 domains. The Raf-MEK-ERK signaling pathway plays vital roles in cell proliferation, differentiation, apoptosis, and migration (), with cellular outcomes depending strongly on the dynamics of activation (33–35).Although Raf1 and the upstream activator … iron oxide used forWebNov 29, 2024 · Here, an optical tweezer-assisted pool-screening and single-cell isolation (OPSI) system is established for precise, indexed isolation of individual bacterial, yeast or human-cancer cells. A controllable static flow field that acts as a cell pool is achieved in a microfluidics chip, to enable precise and ready screening of cells of 1 to 40 μm ... iron oxide yellow nfWebMar 1, 2024 · Single cell screening approaches for antibody discovery Authors Valerie Fitzgerald 1 , Paul Leonard 2 Affiliations 1 Vaccinogen Inc., 949 Fell Street, 2nd Floor, Baltimore, MD 21231, United States. 2 Vaccinogen Inc., 949 Fell Street, 2nd Floor, Baltimore, MD 21231, United States. Electronic address: [email protected]. PMID: … port renfrew bc v0sWebfluidic chip, an optical time-stretch quantitative phase microscope, and a digital image processor equipped with machine learning. As a result, it provides both the opacity and phase maps of every single cell at a high throughput of 10,000 cells/s, enabling accurate cell classification without the need for fluorescent labeling. iron padded folding benchWebDec 23, 2024 · December 23, 2024 Optical 'tweezer' enables fast, low-cost screening of bacteria and cancer cells by Li Yuan, Chinese Academy of Sciences Versatile, facile and low-cost single-cell... port renfrew fishing report 2021WebDec 23, 2024 · An artificial test mixture of green-fluorescent protein (GFP) E. coli, non-GFP E. coli and yeast were loaded onto the chip in a 1:1:1 ratio, and quickly the GFP bacteria and yeast were separated ... iron pack baler