WebOur lab often clones shRNA in pSicoR-derived vectors. Our most commonly used one has cloning sites downstream of a mouse U6 promoter, A 5 BstXI site and a 3 XhoI or BamHI site. In order to clone shRNAs in pSicoR-vectors, we prepare linearized vector by digesting with the appropriate restriction enzymes followed by gel extraction. WebExpert Gene Knockdown You Can Count On. As the exclusive provider of the trusted TRC1.5 and TRC2 collections, our comprehensive MISSION ® shRNA product offering consists of …
Short Hairpin (shRNA) Cloning Vectors OriGene
WebDec 8, 2010 · The stable clones were generated by pHuSH 29mer shRNA constructs against MMP1 (#TR311450 RNAi targeting vector system OriGene Rockville, MD) as described . We were provided with four different sh RNA sequences and we used two different sequences to generate T6-4 and its respective control clone and the second independent clone T6-7 … WebJun 15, 2012 · Cloning—if the resulting double-stranded DNA fragment will be relatively long (>60 bp), or will be used in cloning, we recommend starting with PAGE-purified oligos (IDT can provide this service). Annealing RNA—the IDT research team also uses this protocol to create siRNA duplexes from single-stranded, complementary RNA oligos. smart assessor user engagement academy
The RNAi Consortium shRNA Library Broad Institute
http://www.tinhangtech.com/home/index.php/products/producthighlight/item/14-sigma-aldrich-mission-shrna?tmpl=component&print=1 WebClick pricing for “Bacterial Glycerol Stock” for either the human or mouse for the list of available clones in the Sigma library. Use the TRCN# to search the Vector Core list for … WebThe RNAi Consortium, or TRC, is a public-private effort based at the Broad whose mission is to create a shRNA library as well to validate tools and methods that will enable the … smart assessor wakefield college