Webbof transgenic mouse lines expressing GCaMP6s and GCaMP6f GECIs under the Thy1 promoter [20,21,22]. We characterize the brain-wide expression patterns of each line, and the performance of selected lines for cellular in vivo imaging. Materials and Methods All surgical and experimental procedures were in accordance Webb24 maj 2024 · We hypothesized that VNS would trigger widespread cortical activation that may be observed using widefield GCaMP6s imaging. Using one-photon epifluorescence widefield imaging in Thy1-GCaMP6s mice, which express GCaMP6s throughout excitatory neurons in the cerebral cortex (see Salkoff et al. 24), we were able to quantify the extent …

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WebbSignificance: In vivo imaging and electrophysiology are powerful tools to explore neuronal function that each offer unique complementary information with advantages and … WebbThy1 -GCaMP6-WPRE transgenic mice were generated using standard techniques [23].We included the WPRE (Woodchuck hepatitis virus post-transcriptional regulatory element), … dln integrated systems wyoming mi

Imaging neural activity using Thy1-GCaMP transgenic mice

WebbJanuary 7th, 2024 We describe an experimental procedure for measuring neuronal activity through dual optical windows above bilateral primary somatosensory corticies (S1) in … Webb1 okt. 2024 · (A) Emission spectra of GCaMP6s overlaid with spectra of light passing through the blue filter (FESH0500, 392–497 nm), through the green filter (FBH520–40, 503–539 nm) located before PMT for acquisition of GCaMP6s fluorescence during Ca 2+ imaging and with spectra of white light (380–730 nm) coming from the CRT screen. (B) … Webb12 juli 2024 · In Thy1 -GCaMP6s mice, GCaMP expression was observed in sensory neurons in a variety of sizes (Fig. 4e, f ). We found that the majority of neurons showing … crazy skate cushions